mirror of
https://github.com/deepmodeling/Uni-Lab-OS
synced 2026-03-24 05:48:20 +00:00
修改tracker的问题,在volumetracker报错时,禁用并进行下一步
This commit is contained in:
@@ -10,6 +10,7 @@ from pylabrobot.liquid_handling import LiquidHandler, LiquidHandlerBackend, Liqu
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from pylabrobot.liquid_handling.liquid_handler import TipPresenceProbingMethod
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from pylabrobot.liquid_handling.liquid_handler import TipPresenceProbingMethod
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from pylabrobot.liquid_handling.standard import GripDirection
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from pylabrobot.liquid_handling.standard import GripDirection
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from pylabrobot.resources.errors import TooLittleLiquidError, TooLittleVolumeError
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from pylabrobot.resources.errors import TooLittleLiquidError, TooLittleVolumeError
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from pylabrobot.resources.volume_tracker import no_volume_tracking
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from pylabrobot.resources import (
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from pylabrobot.resources import (
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Resource,
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Resource,
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TipRack,
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TipRack,
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@@ -213,60 +214,60 @@ class LiquidHandlerMiddleware(LiquidHandler):
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if spread == "":
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if spread == "":
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spread = "custom"
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spread = "custom"
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def _safe_aspirate_volumes(_resources: Sequence[Container], _vols: List[float]) -> List[float]:
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for res in resources:
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"""将 aspirate 体积裁剪到源容器当前液量及 tip 剩余容量范围内,避免 volume tracker 报错。"""
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tracker = getattr(res, "tracker", None)
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safe: List[float] = []
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if tracker is None or getattr(tracker, "is_disabled", False):
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_head_owner = getattr(self, '_simulate_handler', None) or self
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continue
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_head = getattr(_head_owner, 'head', {})
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history = getattr(tracker, "liquid_history", None)
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_channels = use_channels or list(range(len(_resources)))
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if tracker.get_used_volume() <= 0 and isinstance(history, list) and len(history) == 0:
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for i, (res, vol) in enumerate(zip(_resources, _vols)):
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fill_vol = tracker.max_volume if tracker.max_volume > 0 else 50000
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req = max(float(vol), 0.0)
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tracker_disabled = False
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try:
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try:
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tracker = getattr(res, "tracker", None)
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tracker.add_liquid(fill_vol)
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tracker_disabled = bool(getattr(tracker, "is_disabled", False))
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if not tracker_disabled:
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get_used = getattr(tracker, "get_used_volume", None)
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if callable(get_used):
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used_volume = get_used()
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if isinstance(used_volume, (int, float)) and used_volume > 0:
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req = min(req, float(used_volume))
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except Exception:
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except Exception:
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pass
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tracker.liquid_history.append(("auto_init", fill_vol))
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if not tracker_disabled:
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try:
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ch = _channels[i] if i < len(_channels) else i
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if ch in _head and _head[ch].has_tip:
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_tip = _head[ch].get_tip()
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_tip_free = _tip.maximal_volume - _tip.tracker.get_used_volume()
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if _tip_free >= 0:
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req = min(req, _tip_free)
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except Exception:
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pass
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safe.append(req)
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return safe
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actual_vols = _safe_aspirate_volumes(resources, vols)
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if actual_vols != vols and hasattr(self, "_ros_node") and self._ros_node is not None:
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self._ros_node.lab_logger().warning(f"[aspirate] volume adjusted, requested_vols={vols}, actual_vols={actual_vols}")
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if self._simulator:
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if self._simulator:
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return await self._simulate_handler.aspirate(
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try:
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resources,
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return await self._simulate_handler.aspirate(
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actual_vols,
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resources,
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use_channels,
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vols,
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flow_rates,
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use_channels,
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offsets,
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flow_rates,
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liquid_height,
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offsets,
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blow_out_air_volume,
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liquid_height,
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spread,
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blow_out_air_volume,
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**backend_kwargs,
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spread,
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)
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**backend_kwargs,
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)
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except (TooLittleLiquidError, TooLittleVolumeError) as e:
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tracker_info = []
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for r in resources:
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t = r.tracker
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tracker_info.append(
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f"{r.name}(used={t.get_used_volume():.1f}, "
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f"free={t.get_free_volume():.1f}, max={r.max_volume})"
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)
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if hasattr(self, "_ros_node") and self._ros_node is not None:
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self._ros_node.lab_logger().warning(
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f"[aspirate] volume tracker error, bypassing tracking. "
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f"error={e}, vols={vols}, trackers={tracker_info}"
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)
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with no_volume_tracking():
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return await self._simulate_handler.aspirate(
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resources,
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vols,
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use_channels,
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flow_rates,
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offsets,
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liquid_height,
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blow_out_air_volume,
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spread,
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**backend_kwargs,
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)
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try:
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try:
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await super().aspirate(
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await super().aspirate(
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resources,
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resources,
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actual_vols,
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vols,
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use_channels,
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use_channels,
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flow_rates,
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flow_rates,
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offsets,
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offsets,
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@@ -279,7 +280,7 @@ class LiquidHandlerMiddleware(LiquidHandler):
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if "Resource is too small to space channels" in str(e) and spread != "custom":
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if "Resource is too small to space channels" in str(e) and spread != "custom":
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await super().aspirate(
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await super().aspirate(
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resources,
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resources,
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actual_vols,
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vols,
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use_channels,
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use_channels,
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flow_rates,
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flow_rates,
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offsets,
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offsets,
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@@ -290,35 +291,15 @@ class LiquidHandlerMiddleware(LiquidHandler):
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)
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)
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else:
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else:
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raise
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raise
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except TooLittleLiquidError:
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# 再兜底一次:按实时可用液量重算后重试,避免状态更新竞争导致的瞬时不足
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retry_vols = _safe_aspirate_volumes(resources, actual_vols)
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if any(v > 0 for v in retry_vols):
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await super().aspirate(
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resources,
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retry_vols,
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use_channels,
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flow_rates,
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offsets,
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liquid_height,
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blow_out_air_volume,
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spread,
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**backend_kwargs,
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)
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actual_vols = retry_vols
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else:
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actual_vols = retry_vols
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res_samples = []
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res_samples = []
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res_volumes = []
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res_volumes = []
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# 处理 use_channels 为 None 的情况(通常用于单通道操作)
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if use_channels is None:
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if use_channels is None:
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# 对于单通道操作,推断通道为 [0]
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channels_to_use = [0] * len(resources)
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channels_to_use = [0] * len(resources)
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else:
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else:
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channels_to_use = use_channels
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channels_to_use = use_channels
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for resource, volume, channel in zip(resources, actual_vols, channels_to_use):
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for resource, volume, channel in zip(resources, vols, channels_to_use):
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sample_uuid_value = getattr(resource, "unilabos_extra", {}).get(EXTRA_SAMPLE_UUID, None)
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sample_uuid_value = getattr(resource, "unilabos_extra", {}).get(EXTRA_SAMPLE_UUID, None)
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res_samples.append({"name": resource.name, "sample_uuid": sample_uuid_value})
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res_samples.append({"name": resource.name, "sample_uuid": sample_uuid_value})
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res_volumes.append(volume)
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res_volumes.append(volume)
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@@ -365,17 +346,43 @@ class LiquidHandlerMiddleware(LiquidHandler):
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actual_vols = _safe_dispense_volumes(resources, vols)
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actual_vols = _safe_dispense_volumes(resources, vols)
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if self._simulator:
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if self._simulator:
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return await self._simulate_handler.dispense(
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try:
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resources,
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return await self._simulate_handler.dispense(
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actual_vols,
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resources,
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use_channels,
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actual_vols,
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flow_rates,
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use_channels,
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offsets,
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flow_rates,
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liquid_height,
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offsets,
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blow_out_air_volume,
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liquid_height,
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spread,
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blow_out_air_volume,
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**backend_kwargs,
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spread,
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)
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**backend_kwargs,
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)
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except (TooLittleLiquidError, TooLittleVolumeError) as e:
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tracker_info = []
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for r in resources:
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t = r.tracker
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tracker_info.append(
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f"{r.name}(used={t.get_used_volume():.1f}, "
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f"free={t.get_free_volume():.1f}, max={r.max_volume})"
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)
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if hasattr(self, "_ros_node") and self._ros_node is not None:
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self._ros_node.lab_logger().warning(
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f"[dispense] volume tracker error, bypassing tracking. "
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f"error={e}, vols={actual_vols}, trackers={tracker_info}"
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)
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with no_volume_tracking():
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return await self._simulate_handler.dispense(
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resources,
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actual_vols,
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use_channels,
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flow_rates,
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offsets,
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liquid_height,
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blow_out_air_volume,
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spread,
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**backend_kwargs,
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)
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try:
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try:
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await super().dispense(
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await super().dispense(
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resources,
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resources,
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@@ -859,6 +866,14 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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local = cast(Union[Container, TipRack], plr)
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local = cast(Union[Container, TipRack], plr)
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if hasattr(plr, "unilabos_extra") and hasattr(local, "unilabos_extra"):
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if hasattr(plr, "unilabos_extra") and hasattr(local, "unilabos_extra"):
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local.unilabos_extra = getattr(plr, "unilabos_extra", {}).copy()
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local.unilabos_extra = getattr(plr, "unilabos_extra", {}).copy()
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if local is not plr and hasattr(plr, "tracker") and hasattr(local, "tracker"):
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local_tracker = local.tracker
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plr_tracker = plr.tracker
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local_history = getattr(local_tracker, "liquid_history", None)
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plr_history = getattr(plr_tracker, "liquid_history", None)
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if (isinstance(local_history, list) and len(local_history) == 0
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and isinstance(plr_history, list) and len(plr_history) > 0):
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local_tracker.liquid_history = list(plr_history)
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resolved.append(local)
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resolved.append(local)
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if len(resolved) != len(uuids):
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if len(resolved) != len(uuids):
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raise ValueError(
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raise ValueError(
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@@ -868,8 +883,18 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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resolved = _resolve_from_local_by_uuids()
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resolved = _resolve_from_local_by_uuids()
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result = list(items)
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result = list(items)
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for (idx, _), plr in zip(dict_items, resolved):
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for (idx, orig_dict), res in zip(dict_items, resolved):
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result[idx] = plr
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if isinstance(orig_dict, dict) and hasattr(res, "tracker"):
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tracker = res.tracker
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local_history = getattr(tracker, "liquid_history", None)
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if isinstance(local_history, list) and len(local_history) == 0:
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data = orig_dict.get("data") or {}
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dict_history = data.get("liquid_history")
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if isinstance(dict_history, list) and len(dict_history) > 0:
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tracker.liquid_history = [
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(name, float(vol)) for name, vol in dict_history
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]
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result[idx] = res
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return result
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return result
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@classmethod
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@classmethod
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@@ -1070,7 +1095,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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for _ in range(len(sources)):
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for _ in range(len(sources)):
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tip = []
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tip = []
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for __ in range(len(use_channels)):
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for __ in range(len(use_channels)):
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tip.extend(self._get_next_tip())
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tip.append(self._get_next_tip())
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await self.pick_up_tips(tip)
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await self.pick_up_tips(tip)
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await self.aspirate(
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await self.aspirate(
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resources=[sources[_]],
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resources=[sources[_]],
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@@ -1110,7 +1135,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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for i in range(0, len(sources), 8):
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for i in range(0, len(sources), 8):
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tip = []
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tip = []
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for _ in range(len(use_channels)):
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for _ in range(len(use_channels)):
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tip.extend(self._get_next_tip())
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tip.append(self._get_next_tip())
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await self.pick_up_tips(tip)
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await self.pick_up_tips(tip)
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current_targets = waste_liquid[i : i + 8]
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current_targets = waste_liquid[i : i + 8]
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current_reagent_sources = sources[i : i + 8]
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current_reagent_sources = sources[i : i + 8]
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@@ -1204,7 +1229,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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for _ in range(len(targets)):
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for _ in range(len(targets)):
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tip = []
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tip = []
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for x in range(len(use_channels)):
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for x in range(len(use_channels)):
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tip.extend(self._get_next_tip())
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tip.append(self._get_next_tip())
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await self.pick_up_tips(tip)
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await self.pick_up_tips(tip)
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await self.aspirate(
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await self.aspirate(
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@@ -1256,7 +1281,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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for i in range(0, len(targets), 8):
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for i in range(0, len(targets), 8):
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tip = []
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tip = []
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for _ in range(len(use_channels)):
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for _ in range(len(use_channels)):
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tip.extend(self._get_next_tip())
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tip.append(self._get_next_tip())
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await self.pick_up_tips(tip)
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await self.pick_up_tips(tip)
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current_targets = targets[i : i + 8]
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current_targets = targets[i : i + 8]
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current_reagent_sources = reagent_sources[i : i + 8]
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current_reagent_sources = reagent_sources[i : i + 8]
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@@ -1538,7 +1563,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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delays = kwargs.get('delays')
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delays = kwargs.get('delays')
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tip = []
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tip = []
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tip.extend(self._get_next_tip())
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tip.append(self._get_next_tip())
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await self.pick_up_tips(tip)
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await self.pick_up_tips(tip)
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blow_out_air_volume_before_vol = 0.0
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blow_out_air_volume_before_vol = 0.0
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if blow_out_air_volume_before is not None and len(blow_out_air_volume_before) > 0:
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if blow_out_air_volume_before is not None and len(blow_out_air_volume_before) > 0:
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@@ -1741,9 +1766,13 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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def iter_tips(self, tip_racks: Sequence[TipRack]) -> Iterator[Resource]:
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def iter_tips(self, tip_racks: Sequence[TipRack]) -> Iterator[Resource]:
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"""Yield tips from a list of TipRacks one-by-one until depleted."""
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"""Yield tips from a list of TipRacks one-by-one until depleted."""
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for rack in tip_racks:
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for rack in tip_racks:
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for tip in rack:
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if isinstance(rack, TipSpot):
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yield tip
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yield rack
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# raise RuntimeError("Out of tips!")
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elif hasattr(rack, "get_all_items"):
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yield from rack.get_all_items()
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else:
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for tip in rack:
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yield tip
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def _get_next_tip(self):
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def _get_next_tip(self):
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"""从 current_tip 迭代器获取下一个 tip,耗尽时抛出明确错误而非 StopIteration"""
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"""从 current_tip 迭代器获取下一个 tip,耗尽时抛出明确错误而非 StopIteration"""
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